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KMID : 0368420080510050347
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Journal of Plant Biology
2008 Volume.51 No. 5 p.347 ~ p.353
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Expression of a Chinese cabbage cysteine proteinase inhibitor, BrCYS1, retards seed germination and plant growth in transgenic Tobacco plant
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Hong Joon-Ki
Hwang Jung-Eun
Chung Woo-Sik
Lee Kyun-Oh
Choi Young-Ju
Gal Sang-Wan
Park Beom-Seok
Lim Chae-Oh
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Abstract
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Phytocystatins are plant cysteine proteinase inhibitors that regulate endogenous and heterologous cysteine proteinases of the papain family. A cDNA encoding the phytocystatin BrCYS1 (Brassica rapa cysteine proteinase inhibitor 1 ) has been isolated from Chinese cabbage (B. rapa subsp.pekinensis) flower buds. In order to explore the role of this inhibitory enzyme, tobacco plants (Nicotiana tabacum L. cv. Samson) containing altered amounts of phytocystatin were generated by over-expressingBrCYS1 cDNA in either the sense or the antisense configuration. The resulting plants hadin vitro enzyme inhibitory activities that were over 10% of those detected in wild type plants. The transgenic plants exhibited retarded seed germination and seedling growth and a reduced seed yield, whereas these properties were enhanced in antisense plants. These data suggest that BrCYS1 participates in the control of seed germination, post-germination and plant growth by regulating cysteine peptidase activity.
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KEYWORD
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cysteine proteinase, gene expression, inhibitory activity, phytocystatin, sense and antisense, transformation
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